| First String | Second String | Third/Fourth String | |
| QB | Brett Favre | Tarvaris Jackson | Sage Rosenfels |
| RB | Adrian L. Peterson | Toby Gerhart | Ryan Moats | Albert Young |
| FB | Naufahu Tahi | ||
| RWR | Sidney Rice | Greg Lewis | Freddie Brown |
| LWR | Bernard Berrian | Percy Harvin | Jaymar Johnson | Joe Webb |
| TE | Visanthe Shiancoe | Jim Kleinsasser | Mickey Shuler | Jeff Dugan |
| ORT | Phil Loadholt | Ryan Cook | |
| ORG | Anthony Herrera | Chris Degeare | |
| C | John Sullivan | Jon Cooper | |
| OLG | Steve Hutchinson | ||
| OLT | Bryant McKinnie | Ryan Cook | Patrick Brown |
| LDE | Ray Edwards | Everson Griffen | Mike Montgomery |
| LDT | Pat Williams | Fred Evans | |
| RDT | Kevin Williams | Jimmy Kennedy | Letroy Guion |
| RDE | Jared Allen | Brian Robison | |
| SSL | Chad Greenway | Heath Farewell | |
| ML | E.J. Henderson | Jasper Brinkley |
Nate Triplett |
| WSL | Ben Leber | Erin Henderson | |
| LCB | Antoine Winfield | Chris Cook | Benny Sapp |
| RCB | Cedric Griffin | Lito Sheppard | Asher Allen |
| SS | Tyrell Johnson | Jamarca Sanford | Eric Frampton |
| FS | Madieu Williams | Husain Abdullah | |
| PK | Ryan Longwell | Rhys Lloyd | |
| P | Chris Kluwe | ||
| LS | Cullen Loeffler | ||
| H | Chris Kluwe | ||
| KR | Percy Harvin | Jaymar Johnson | Darius Reynaud |
| PR | Jaymar Johnson | Percy Harvin | Bernard Berrian |